Amalasuntha (also known as Amalasuentha, Amalaswintha or Amalasuintha) (d. 535) was a queen of the Ostrogoths.

A daughter of Ostrogothic king Theodoric the Great, she secretly married a slave named Traguilla. When her mother Audofleda found them together Traguilla was killed.

She was married in 515 to Eutharic, an Ostrogoth noble of the old Areal line, who had previously been living in Visigothic Iberia. Her husband died, apparently in the early years of her marriage, leaving her with two children, Athalaric and Matasuentha. On the death of her father in 526, her son succeeded him, but she held the power as regent for her son. Deeply imbued with the old Roman culture, she gave to that son's education a more refined and literary turn than suited the ideas of her Gothic subjects. Conscious of her unpopularity she banished, and afterwards put to death, three Gothic nobles whom she suspected of plotting against her rule, and at the same time opened negotiations with the emperor Justinian I with the view of removing herself and the Gothic treasure to Constantinople. Her son's death in 534 made little change in the posture of affairs.

Now queen, Amalasuntha made her cousin Theodahad partner of her throne (not, as sometimes stated, her husband, for his wife was still living), with the intent of strengthening her position. The choice was unfortunate, for Theodahad, in spite of a varnish of literary culture, was a coward and a scoundrel.

Her friendly relations with the Byzantine emperor Justinian I alienated her people. In 535 the Ostrogoths revolted; Amalasuntha was exiled to an island in the Tuscan lake of Bolsena, where in the spring of 535 she was murdered in her bath. Justinian used her murder as his pretext for attacking and reconquering Italy.

The letters of Cassiodorus, chief minister and literary adviser of Amalasuntha, and the histories of Procopius and Jordanes, give us our chief information as to the character of Amalasuntha.